Multiple resistance to sulfonylureas and imidazolinones conferred by an...
SummaryThe acetohydroxyacid synthase (AHAS) gene from the Arabidopsis thaliana mutant line GH90 carrying the imidazolinone resistance allele imr1 was cloned. Expression of the AHAS gene under the...
View ArticleMolecular characterization of plastid pyruvate kinase from castor and tobacco
AbstractClones encoding two different forms of plastid pyruvate kinase (PKp; EC 2.7.1.40) have been isolated from both castor and tobacco seed cDNA libraries. One form, designated PKpA, from castor was...
View ArticleAssessment of microinjection for introducing DNA into uninuclear microspores...
AbstractApproximately 2,000 embryogenic uninuclear microspores of rapeseed (Brassica napus) cv. Topas were intranuclearly injected with a chimaeric β-glucuronidase (Escherichia coli Uid A) gene. Stable...
View ArticleAn acetohydroxy acid synthase mutant reveals a single site involved in...
AbstractAcetohydroxy acid synthase (AHAS) is an essential enzyme for many organisms as it catalyzes the first step in the biosynthesis of the branched-chain amino acids valine, isoleucine, and leucine....
View ArticleFunctional analysis of a RPD3 histone deacetylase homologue in Arabidopsis...
AbstractHistone acetylation is modulated through the action of histone acetyltransferase and deacetylase, which play key roles in the regulation of eukaryotic gene expression. We have screened the...
View ArticleTransgene expression and control
SummaryThe relative ease of producing transgenic plants is an enormous experimental advantage for studies into the organization, function and evolution of plant genes and their expression systems....
View ArticleControl of Gene Expression by Histone Deacetylases
AbstractIn eukaryotes, the strength of the interactions between the basic tails of the core histones and DNA can be modulated through post-translational modifications, such as acetylation (Strahl and...
View ArticleThe cryptic enhancer elements of the tCUP promoter
AbstractExamination of the tCUP cryptic promoter from tobacco demonstrates that cryptic gene regulatory elements in the plant genome are functionally equivalent to elements responsible for the...
View ArticleActivity of elements from the tobacco cryptic promoter, tCUP , in conifer...
SummaryCryptic gene regulatory elements are inactive at their native locations in the genome but are fully functional when positioned adjacent to genes in transgenic plants. Although they are...
View ArticleCHB2 , a member of the SWI3 gene family, is a global regulator in Arabidopsis
AbstractThe SWI/SNF complex is an ATP-dependent chromatin remodeling complex that plays an important role in the regulation of eukaryotic gene expression. Very little is known about the function of...
View ArticleRepression of Plant Gene Expression via Chromosomal Remodelling Using Histone...
Posttranscriptional modification of nucleosomal histones is an important mechanism in the regulation of eukaryotic gene expression. Acetylation and deacetylation is a major process of such regulation....
View ArticlepORE: a modular binary vector series suited for both monocot and dicot plant...
AbstractWe present a series of 14 binary vectors suitable for Agrobacterium-mediated transformation of dicotyledonous plants and adaptable for biolistic transformation of monocotyledonous plants. The...
View ArticleTranscriptome analysis reveals absence of unintended effects in...
AbstractBackgroundPlants engineered for abiotic stress tolerance may soon be commercialized. The engineering of these plants typically involves the manipulation of complex multigene networks and may...
View ArticleControl of somatic embryogenesis and embryo development by AP2 transcription...
AbstractMembers of the AP2 family of transcription factors, such as BABY BOOM (BBM), play important roles in cell proliferation and embryogenesis in Arabidopsis thaliana (AtBBM) and Brassica napus...
View ArticleColoring Soybeans with Anthocyanins?
AbstractThe seed coats of black soybean (Glycine max (L.) Merr.) accumulate all anthocyanins required for the red (cyanidin-), blue (delphinidin-), purple (petunidin-), and orange...
View ArticleCombined analysis of transcriptome and metabolite data reveals extensive...
AbstractBackgroundThe R locus controls the color of pigmented soybean (Glycine max) seeds. However information about its control over seed coat biochemistry and gene expressions remains limited. The...
View ArticleRole of HD2 genes in seed germination and early seedling growth in Arabidopsis
AbstractThe Arabidopsis HD2 family of histone deacetylases consist of 4 members (HD2A, HD2B, HD2C, HD2D) that play diverse roles in plant development and physiology through chromatin remodelling. Here,...
View ArticleColoring genetically modified soybean grains with anthocyanins by suppression...
AbstractDetection and quantification of the levels of adventitious presence of genetically modified (GM) soybeans in non-GM grain shipments currently requires sophisticated tests that can have issues...
View ArticleComparability of imazapyr-resistant Arabidopsis created by transgenesis and...
AbstractThe Arabidopsis CSR1 gene codes for the enzyme acetohydroxyacid synthase (AHAS, EC 2.2.1.6), also known as acetolactate synthase, which catalyzes the first step in branched-chain amino acid...
View ArticleExpression of a fungal ferulic acid esterase in alfalfa modifies cell wall...
AbstractBackgroundAlfalfa (Medicago sativa) is an important forage crop in North America owing to its high biomass production, perennial nature and ability to fix nitrogen. Feruloyl esterase (EC...
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